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  A wide diversity was detected among the collected germplasms at the phenotypic as well as molecular level. Of the 21 SSR markers, 15 markers were found to be polymorphic with 88 alleles. Based on phenotypic and biochemical results, 21 genotypes were identified as salinity tolerant, whereas 40 genotypes turned out to be salt susceptible. The present study shows that apart from the established salt tolerant lines, several other landraces like Bonkanta, Morisal, Ghiosh, Patni may be the source of salt tolerant donor in future breeding programs. © Prof. H.S. Srivastava Foundation for Science and Society 2020.To determine the effects of carboxymethyl chitosan on plant growth and nutrient uptake, Prunus davidiana seedlings were treated with various concentrations of carboxymethyl chitosan. The biomass, physiological characteristics, and nutrient uptake of the treated P. davidiana seedlings were then examined. Compared with the control seedlings, the carboxymethyl chitosan-treated seedlings had a higher biomass and a greater abundance of photosynthetic pigments (i.e., chlorophyll a, chlorophyll b, total chlorophyll, and carotenoid), with the best concentration as 2 g/L carboxymethyl chitosan, which increased the shoot biomass and total chlorophyll content by 26.75% and 24.64%, respectively. Moreover, the application of carboxymethyl chitosan enhanced superoxide dismutase and catalase activities, increased the soluble protein content, and decreased the malondialdehyde and proline contents of the P. davidiana seedlings to some extent. Additionally, the carboxymethyl chitosan treatments decreased the total nitrogen content, but increased the total phosphorus and potassium contents in P. davidiana seedlings to some extent. The minimum of total nitrogen content and the maximum of total phosphorus and potassium contents in shoots of P. davidiana seedlings were the concentration of 2 g/L carboxymethyl chitosan, which was decreased by 12.96% and increased by 15.45% and 22.53%, respectively, compared with the control. Therefore, the application of a carboxymethyl chitosan solution may promote the growth, enhance the stress resistance, and alter the nutrient uptake of P. davidiana seedlings, especially at 2 g/L carboxymethyl chitosan. © Prof. H.S. Srivastava Foundation for Science and Society 2020.Market is increasingly demanding vegetables with high quality and nutraceutical characteristics. It was demonstrated that leafy vegetables can get benefit from biostimulants, for the reduction of nitrate concentration and the increment of antioxidants, with potential benefit for human health. The research purpose was to investigate on the role of a novel plant-based biostimulant in affecting nitrogen and carbon metabolism in wild rocket (Diplotaxis tenuifolia L.). Foliar spray treatments were performed with extracts obtained from borage (Borago officinalis L.) leaves and flowers. To evaluate the treatments effect, in vivo determinations (chlorophyll a fluorescence and chlorophyll content) were performed. At harvest, nitrate concentration, sucrose, total sugars, chlorophyll, and carotenoids levels were measured in leaves. In order to characterize the mechanism of action also at molecular level, a set of genes encoding for some of the key enzymes implicated in nitrate and carbon metabolism was selected and their expression was measured by qRT-PCR. Interesting results concerned the increment of sucrose, coherent with a high value of Fv/Fm, in addition to a significant reduction of nitrate and ABA than control, and an enhanced NR in vivo activity. Also, genes expression was influenced by extracts, with a more pronounced effect on N related genes. © Prof. H.S.  https://www.selleckchem.com/products/LBH-589.html  Srivastava Foundation for Science and Society 2020.In plants, previous studies show that telomerase activity contributes to the maintenance of telomeric length for the proper development of organs and tissues. In this work, we investigated telomerase activity in A. tequilana during several years of cultivation. We found that during growth of the leaf there are two crucial phases (1) the onset of cell elongation in 3 years and (2) differentiation of vascular bundles in 6 years. This coincides with the ages where the highest telomerase activity is seen. Therefore indicates that telomerase is associated with cellular activities such as; elongation, division, and cell differentiation. Likewise, we detected high activity during the period of vegetative growth, indicating that telomerase also contributes to telomeric maintenance on the leaf in A. tequilana. © Prof. H.S. Srivastava Foundation for Science and Society 2020.The fruit is the most important economical organ in the grape; accordingly, to investigate the grapevine genomic methylation landscape and examine its functional significance during fruit development, we generated whole genome DNA methylation maps for various developmental stages in the fruit of grapevine. In this study, thirteen DNA methylation-related genes and their expression profiles were identified and analyzed. The methylation levels for mC, mCG, mCHG, and mCHH contexts in 65 days after flowering (65DAF) fruit (véraison stage) were higher than those in 40DAF (green stage) and 90DAF (mature stage) fruits. Relative to methylation in the mC context, methylation levels in the mCHH context were higher than those of mCG and mCHG. The DNA methylation level in the ncRNA regions was significantly higher than that in exon, gene, intron, and mRNA regions. The differentially methylated regions (DMRs) and differentially methylated promoters (DMPs) in 65DAF_vs_40DAF were both higher than those in 90DAF_vs_65DAF and 90DAF_vs_40DAF. Most DMRs (or DMPs) were involved in metabolic processes and cell processes, binding, and catalytic activity. These results indicated that DNA methylation represses gene expression during grape fruit development, and it broadens our understanding of the landscape and function of DNA methylation in grapevine genomes.  https://www.selleckchem.com/products/LBH-589.html  © Prof. H.S. Srivastava Foundation for Science and Society 2020.The upgrade to biomethane allows extending biogas applications to transport sectors, supporting EU goals toward carbon neutrality. Biomethane produced from biogas upgrading can today rely on a large number of plants, estimated by the European Biogas Association in over 17000 in 2016, for a total installed capacity of 9985 GW (EBA, 2017). After 2020, biogas and biomethane will count towards the 32% target of renewable energy share of the EU energy consumption, and towards a sub-target of minimum 14% of the energy consumed in the transport sector by 2030 (REDII). In this framework, the paper aims to define the current market penetration of biogas upgrading technologies. A database has been created for EU-28, to highlight the relative importance of each technology. Based on the database, a EU production potential is defined, along with the EU demand potential for transport, in order to verify the match between supply and demand. The analysis of the current state of play of the sector suggests that a large potential can be foreseen for near future; the expected increase in biomethane production is based also on the possibility to convert residual feedstocks, such as Municipal Solid Wastes.