001). ROC curve analysis revealed that at a threshold of 3,430 IU/mL (Log 3.54), qPCR had a sensitivity of 97.06% and a specificity of 74.24% (AUC 0.92617 ± 0.0185, p<0.001), in the prediction of 10 cells/105 leukocytes by antigenemia and physician's decision to treat.
 
  CMV Pp65 antigenemia and CMV qPCR showed fair agreement and a moderate correlation in this study. The in-house qPCR was revealed to be an accurate method to determine CMV DNAemia in kidney transplant patients, resulting in positive results weeks before antigenemia.
 CMV Pp65 antigenemia and CMV qPCR showed fair agreement and a moderate correlation in this study. The in-house qPCR was revealed to be an accurate method to determine CMV DNAemia in kidney transplant patients, resulting in positive results weeks before antigenemia.
  Cryopreservation is an effective tool for the preservation of live biological materials.
 
  This study examined the suitability of cryopreservation protocols and the effectiveness of ultrasound for silver carp embryos.
 
  Embryos at three developmental stages were exposed to 10, 15, 20, and 25% of five cryoprotectants (CPAs), namely propylene glycol (PG), dimethylformamide (DFA), DMSO, MeOH, and ethylene glycol (EG) for 20 min. Embryos were exposed to twelve vitrification solutions (VSs) for 10 (five steps of 2 min), 15 (five steps of 3 min), 20 (five steps of 4 min) min.  https://www.selleckchem.com/products/mpi-0479605.html  Embryos were also exposed to ultrasound in VSs prior to cooling for cryopreservation.
 
  Hatching rates decreased with increasing CPA concentrations while toxicity varied in the order of PG < DMSO < EG < MeOH < DFA. Tail elongation stage was more tolerant to CPA than 6-somites and morula stages. The survival of embryos exposed to ultrasound in VS was remarkably lower than in water. Embryos exposed to ultrasound in VSs under the best conditions did not response well after attempted vitrification.
 
  Ultrasound-mediated CPA impregnation could be effective but other innovative methods may be needed to attain successful cryopreservation.
 Ultrasound-mediated CPA impregnation could be effective but other innovative methods may be needed to attain successful cryopreservation.
  Cryopreservation is a reliable and economical method for the long-term ex situ conservation of valuable genetic resources.
 
  The present study focuses on establishing novel regeneration strategies and on assessing various cryogenic methods using nodal explants/shoot apices and on developing in vitro technologies for germplasm conservation of Dioscorea prazeri.
 
  Pre-treatment, growth regulators, temperature conditions, treatment period for recovery and growth of explants were optimized and various germplasm conservation methods were conducted to attain the conservation and mass multiplication of the endangered therapeutic plant. The plants regenerated from vitrified tissues were evaluated for physiological stability through morphological characteristics, genetic stability using RAPD analysis and with key metabolites for biochemical characterization.
 
  An optimized vitrification method resulted in a regeneration level of 92 ± 2 %, whereas a method comprising encapsulation dehydration resulted in 75 ± 2 % regeneration. In contrast, only a 38 ± 2 % regeneration was achieved using an encapsulation vitrification method.
 
  Vitrification-based procedures significantly improve cryopreservation survival and can be successfully employed for the long-term conservation of Dioscorea species and, potentially, other medicinal plants.
 Vitrification-based procedures significantly improve cryopreservation survival and can be successfully employed for the long-term conservation of Dioscorea species and, potentially, other medicinal plants.
  The seeds of oak (Quercus) species cannot be conserved in conventional seed banks and require cryobiotechnologies for long-term, ex situ conservation.
 
  To evaluate shoot tip cryopreservation as a method for conserving oaks ex situ.
 
  Droplet vitrification was tested on tips from shoot cultures of four oak species; the effects of three preculture and recovery growth conditions on survival and growth were tested with Q. virginiana.
 
  Shoot tips of Q. virginiana gave the best survival through liquid nitrogen exposure (56%), compared with Q. hinckleyi (20%), Q. suber (12%), and Q. gambelii (0%). In further tests with Q. virginiana, recovery in the alternating temperature regime (27 C / 151C, 16 h / 8 h) gave significantly better survival than at constant 21sC and significantly better growth than constant 26CC.
 
  While there are species differences in the response to droplet vitrification, these results indicate that shoot tip cryopreservation should be explored further as a conservation tool for oaks.
 While there are species differences in the response to droplet vitrification, these results indicate that shoot tip cryopreservation should be explored further as a conservation tool for oaks.
  Cryolipolysis is a non-invasive method capable of reducing the thickness of the fat layer.
 
  To evaluate the effects of cryolipolysis with the use of plate applicators in the treatment of abdominal fat in women.
 
  The sample was composed of 15 participants, who were evaluated before and at the end of the intervention. Three applications of cryolipolysis were performed in the infraumbilical portion of the abdominal region. The volunteers were divided into three groups G-1 (temperature of -2°C), G-2 (temperature of -3°C) and G3 (temperature of -4°C).
 
  There was a reduction in plicometry measurements in groups G2 and G3, in the comparison between the initial and final moments (p <0.05), and a reduction in perimetry and ultrasound (p < 0.05) in all groups. It was found that the G3 group was subject to higher risk of first degree burns and redness when compared to the other groups.
 
  It is suggested that plate cryolipolysis is a possibly effective resource for reducing adiposity, as shown in the evaluation of perimetry, plicometry, and ultrasound results, and in the photographic analysis.
 It is suggested that plate cryolipolysis is a possibly effective resource for reducing adiposity, as shown in the evaluation of perimetry, plicometry, and ultrasound results, and in the photographic analysis.