https://www.selleckchem.com/products/proteinase-k.html 1%, 43.6%, 29.8% and 77.3%, respectively. The sensitivity, specificity, PPV and NPV of UCA ≥110.97 degrees with CL <3.4 centimeters for predicting preterm birth were 48.8%, 68.4%, 36.2% and 78.4%. The specificity and NPV of UCA with CL were higher than for UCA alone. With adequate sensitivity and high NPV, UCA by TVS can be supplemented with CL measurement in threatened preterm labor management to increase the diagnostic performance for predicting preterm birth. With adequate sensitivity and high NPV, UCA by TVS can be supplemented with CL measurement in threatened preterm labor management to increase the diagnostic performance for predicting preterm birth. Significant progress has been made towards achieving the 90-90-90 global targets by 2020. Nationally, 72% of HIV-positive people aged 15-64 years in urban areas were aware of their HIV status. There is low habit of male partner accompanying pregnant women during ANC visit. This study assessed the HIV test service utilization and associated factors among pregnant women's partners in Addis Ababa. A community-based cross-sectional study design was conducted from January to February 2020 in Addis Ababa town. A multistage sampling method was used to recruit 812 participants. Data were entered into EpiData version 4.1 and analysis was done by using SPSS version 25.0. The presence and strength of significant association with outcome variables in multivariable analysis were determined with P<0.05 and at 95% CI, respectively. Among respondents, 63.7% [95% CI (60-67%)] of pregnant woman partners had tested for HIV/AIDS. Knowledge on the route of HIV transmission from mother to child (AOR=1.9, 95% CI (1.3-2.7))in the study area. The knowledge on ways of prevention of HIV from mother to child transmission, those who received couple HIV counseling before, those who discussed HCT at ANC services with a partner, and heard about discordant HIV test results were predictors of