Cd-induced cell survival enhanced by SP600125 but rescued by Z-VAD-FMK or KPT-335. These results suggest that cadmium cytotoxicity on bone involved exportin 1 accumulation, phosphorylation of JNK, induction of DNA damage and pro-apoptosis, which was induced by activation of caspase-dependent pathways.The chemical ecology of rotifers has been little studied. A yet unknown property is presented within some monogonant rotifers, namely the ability to produce an exogenic filamentous biopolymer, named 'Rotimer'. This rotifer-specific viscoelastic fiber was observed in six different freshwater monogonants (Euchlanis dilatata, Lecane bulla, Lepadella patella, Itura aurita, Colurella adriatica and Trichocerca iernis) in exception of four species. Induction of Rotimer secretion can only be achieved by mechanically irritating rotifer ciliate with administering different types (yeast cell skeleton, denatured BSA, epoxy, Carmine or urea crystals and micro-cellulose) and sizes (approx. from 2.5 to 50 µm diameter) of inert particles, as inductors or visualization by adhering particles. The thickness of this Rotimer is 33 ± 3 nm, detected by scanning electron microscope. This material has two structural formations (fiber or gluelike) in nano dimension. The existence of the novel adherent natural product becomes visible by forming a 'Rotimer-Inductor Conglomerate' (RIC) web structure within a few minutes. The RIC-producing capacity of animals, depends on viability, is significantly modified according to physiological- (depletion), drug- (toxin or stimulator) and environmental (temperature, salt content and pH) effects. The E. dilatata-produced RIC is affected by protein disruptors but is resistant to several chemical influences and its Rotimer component has an overwhelming cell (algae, yeast and human neuroblastoma) motility inhibitory effect, associated with low toxicity. This biopolymer-secretion-capacity is protective of rotifers against human-type beta-amyloid aggregates.Microplastics are ubiquitous in aquatic ecosystems, but little information is currently available on the dangers and risks to living organisms.  https://www.selleckchem.com/products/AZD2281(Olaparib).html  In order to assess the ecotoxicity of environmental microplastics (MPs), samples were collected from the beaches of two islands in the Guadeloupe archipelago, Petit-Bourg (PB) located on the main island of Guadeloupe and Marie-Galante (MG) on the second island of the archipelago. These samples have a similar polymer composition with mainly polyethylene (PE) and polypropylene (PP). However, these two samples are very dissimilar with regard to their contamination profile and their toxicity. MPs from MG contain more lead, cadmium and organochlorine compounds while those from PB have higher levels of copper, zinc and hydrocarbons. The leachates of these two samples of MPs induced sublethal effects on the growth of sea urchins and on the pulsation frequency of jellyfish ephyrae but not on the development of zebrafish embryos. The toxic effects are much more marked for samples from the PB site than those from the MG site. This work demonstrates that MPs can contain high levels of potentially bioavailable toxic substances that may represent a significant ecotoxicological risk, particularly for the early life stages of aquatic animals.Recently, the pollution of microplastics (MPs) in the global freshwater environment has become increasingly problematic, but there are few studies on the freshwater environment risks of MPs. The present study, therefore, has investigated the single and combined effects of MPs and lead (Pb) on the freshwater algal Microcystis aeruginosa. Results showed that Pb-only (>0.05 mg·L-1) promoted the growth of algal cells, while MPs-only (1 mg L-1) resulted in growth inhibition. However, compared with the corresponding concentration of Pb-only groups, the growth of algal cells was promoted in MPs + Pb treatments. MPs-only and Pb-only (0.5 mg L-1) both reduced the content of photosynthetic pigments and affected algal photosynthesis. The MPs-only treatment and MPs + Pb2+ (no pretreatment, 0.5 mg L-1 Pb2+) treatments showed significant cell aggregation. At the same time, MPs-only caused a significant increase in bound extracellular polysaccharides (bEPS), while 0.5 mg L-1 Pb reduced bEPS. Furthermore, under high Pb stress (0.5 mg L-1), the effects of combined MPs and Pb on chlorophyll content, antioxidant enzyme activity (peroxidase (POD), catalase (CAT)), and damage to algal cells were less compared to individual effects, and the combination of MPs and Pb had a synergistic effect on promoting aggregations of M. aeruginosa. These results demonstrate that single and combined effects of MPs and Pb can induce differential responses in the freshwater algal M. aeruginosa, which can have a significant impact on aquatic ecosystems.Even after being abandoned for many years, a large number of weathered crust elution-deposited rare earth (WCED-RE) tailings continue to release ammonia nitrogen (AN) pollution into their surrounding environments. However, the influences of particle size distribution and clay minerals on AN pollution caused by these tailings have been insufficiently studied, and its causes are poorly understood. In this study, soil samples at different depths (5, 7, 9, 11 and 14 m) were collected from a rare earth tailing in Ganzhou City, Jiangxi Province, China. Particles were screened by size into six groups (2-1, 1-0.5, 0.5-0.25, 0.25-0.1, 0.1-0.075 and less then 0.075 mm), and AN forms were extracted. The results showed that as soil particle size decreases, both soil specific surface area and clay content increase, leading to stronger AN enrichment ability. With increased sampling depth, the distribution of clay across the six particle fractions became more uniform, such that the accumulation of AN in soil with fine particle size was less obvious. Clay minerals with different capacities for AN enrichment vary with sampling depth. This variation is responsible for the profile of AN distribution in the mine, where AN first increases and then decreases as vertical depth is increased. Although AN content was highest at 11 m, water soluble AN content was higher in the upper part of the completely weathered layer (5 and 7 m), which poses a higher environmental risk. This study provides significant information to deepen our understanding of the distribution characteristics of AN and its main influencing factors, as well as a foundation for the prevention and remediation of nitrogen pollution from WCED-RE tailings.